Gene Expression in Primary Cultured Mouse Hepatocytes with a Cationic Liposomal Vector, TFL-3: Comparison with Rat Hepatocytes
نویسندگان
چکیده
منابع مشابه
Culture time-dependent gene expression in isolated primary cultured rat hepatocytes by transfection with the cationic liposomal vector TFL-3.
The development of a carrier system that enables the transfer of a functional exogenous gene to non- or less frequently dividing mammalian cells is essential for increasing the available options for the treatment of various diseases. The issue of whether TFL-3, a recently developed cationic liposome, can be successfully used to achieve gene expression in primary cultured rat hepatocytes was exa...
متن کاملCholesterol Uptake in Cultured Primary Mouse Hepatocytes
1 Intracellular Cholesterol Binding Proteins Enhance HDL-mediated 2 Cholesterol Uptake in Cultured Primary Mouse Hepatocytes 3 By 4 5 Stephen M. Storey, Avery L. McIntosh, Huan Huang, Kerstin K. Landrock, Gregory G. 6 Martin, Danilo Landrock, H. Ross Payne, Barbara P. Atshaves, Ann B. Kier, and Friedhelm 7 Schroeder 8 9 from the 10 Departments of Physiology and Pharmacology and Pathobiology, 11...
متن کاملBiol. Pharm. Bull. 28(8) 1472—1475 (2005)
cause hepatocytes synthesize a wide variety of proteins, perform a variety of post-translational modifications, and are involved in numerous diseases. Therefore, hepatocyte-targeted gene transfer would represent an important strategy for expanding treatment options for liver diseases. The successful gene expression in mouse hepatocytes in living animals was recently achieved by a rapid injectio...
متن کاملTemporal gene expression analysis of monolayer cultured rat hepatocytes.
The use of cultured primary hepatocytes within toxicology has proven to be a valuable tool for researchers, however, questions remain with regard to functional differences observed in these hepatocytes relative to the intact liver. Cultured hepatocytes have typically been described as dedifferentiated, a classification based upon the investigation of a few key cellular processes or hepatocellul...
متن کاملImproved oxygenation dramatically alters metabolism and gene expression in cultured primary mouse hepatocytes
Primary hepatocyte culture is an important in vitro system for the study of liver functions. In vivo, hepatocytes have high oxidative metabolism. However, oxygen supply by means of diffusion in in vitro static cultures is much less than that by blood circulation in vivo. Therefore, we investigated whether hypoxia contributes to dedifferentiation and deregulated metabolism in cultured hepatocyte...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2005
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.28.1472